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microscopy slide  (Thermo Fisher)


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    Structured Review

    Thermo Fisher microscopy slide
    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
    Microscopy Slide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 72827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscopy slide/product/Thermo Fisher
    Average 99 stars, based on 72827 article reviews
    microscopy slide - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1"

    Article Title: A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1

    Journal: PLOS Genetics

    doi: 10.1371/journal.pgen.1011990

    (A) . Structured Illumination Microscopy (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
    Figure Legend Snippet: (A) . Structured Illumination Microscopy (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.

    Techniques Used: Microscopy, Expressing



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    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
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    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
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    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
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    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
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    (A) . Structured Illumination <t>Microscopy</t> (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.
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    Image Search Results


    (A) . Structured Illumination Microscopy (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.

    Journal: PLOS Genetics

    Article Title: A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1

    doi: 10.1371/journal.pgen.1011990

    Figure Lengend Snippet: (A) . Structured Illumination Microscopy (SIM) images of 8325-4 CxaR-GFP expressing a fluorescent derivative of CxaR from cxaR native chromosomal locus as the sole source of CxaR (left), labelled with fluorescent D-amino acid HADA which is incorporated into the cell wall (middle) and overlay of the two channels (right; CxaR-GFP signal in cyan, cell wall signal in magenta), showing late septal localization of CxaR-GFP. (B-D) SIM images showing CxaR-GFP localization upon deletion of ftsK (B) , deletion of clpX (C) or inactivation of ClpX (D) . CxaR-GFP septal localization is lost in the absence of FtsK or ClpX, while the protein accumulates in foci upon inactivation of ClpX. Representative images from 3 biological replicates. Scale bars 1 μm.

    Article Snippet: Cells were harvested by centrifugation at 10000 rpm for 1 minute, resuspended in 20 μL of PBS and 1μL of this suspension was mounted on a microscopy slide with a 1.2% agarose (TopVision, Thermo Fisher Scientific) in PBS pad.

    Techniques: Microscopy, Expressing